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Habaki's scheme gets Manji trapped in a dungeon. Habaki uses a doctor, Burando Ayame, to try and solve the mystery of the immortality. For the sake of the advancement of medicine, Burando sinks a scalpel into Manji's body. Meanwhile, while waiting for Manji's return, Rin is caught up in a strange cohabitation with Doua and Isaku, who had been searching for a place to stay.
Episodes
1Act One - Meeting 2Act Two - Founding 3Act Three - Dream Pangs 4Act Four - Rin at Odds 5Act Five - Song of the Bugs 6Act Six - Wing Roots 7Act Seven – Evil's Shadow 8Act Eight - Mugai-ryu 9Act Nine - Gathering 10Act Ten - Animal 11Act Eleven – Fall Frost 12Act Twelve – Blood of Finality 13Act Thirteen - Twilight 14Act Fourteen – Ammendments 15Act Fifteen – Acquisition of Guts 16Act Sixteen – Altered Limb 17Act Seventeen – Ceremonial Bond 18Act Eighteen – Banshee's Cries 19Act Nineteen – Massacre 20Act Twenty – Unending 21Act Twenty One – Trap 22Act Twenty Two – Ten Final Pushes 23Act Twenty Three – 100 Spectacular Dances 24Final Act – Blade of the Immortal